全基因组分析揭示了长非编码RNAs 调节Rhizoctonia solani通过大米的代谢途径抵抗
Wei Li1, Yifan Huang1, Yanhui Xia1
1State Key Laboratory of Hybrid Rice, Hubei Hongshan Laboratory, College of Life Science, Wuhan University, Wuhan 430072, China.
Journal of agricultural and food chemistry
|July 1, 2025
概括
这项研究揭示了长非编码RNAs (lncRNAs) 如何调节对破坏性病原体Rhizoctonia solani的米防御,突出了关键的代谢途径和参与植物免疫的酶.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物病理学 植物病理学
- 基因组学就是基因组学.
背景情况:
- 由Rhizoctonia solani引起的米病是大米生产的主要威胁.
- 了解大米对R. solani防御的分子机制对于开发耐药品种至关重要.
- 长非编码RNAs (lncRNAs) 越来越多地被认为是它们在基因调节中的作用,但它们在米病原体相互作用中的功能仍未得到充分探索.
研究的目的:
- 为了识别和描述涉及到防治R. solani. 的IncRNAs.
- 阐明由lncRNAs调节的调节网络,包括cis调节和竞争的内源RNA (ceRNA) 途径.
- 确定关键的酶和代谢途径,对大米免疫至关重要.
主要方法:
- RNA测序以识别对R. solani反应的 lncRNAs.
- 生物信息分析以预测lncRNA-目标mRNA相互作用 (cis调节和ceRNA网络).
- 目标基因的功能丰富分析.
- 基因淘汰实验验验证了植物防御中的酶功能.
- 抗真菌测试用于评估特定代谢物的活性.
主要成果:
- 在大米中鉴定了833个对R. solani有反应的lncRNA,它们被组织成不同的表达模块.
- 预测了551个mRNA对366个lncRNA的点,揭示了648个与代谢途径 (类,斯酸,三) 相关的cis调节关系.
- 发现了40个作为ceRNA的lncRNA,通过22个miRNA调节73个通过代谢丰富的mRNA.
- 鉴定了三个关键的代谢节点 (OsKSL12,OsSANRL.1,OsCYP87C2),涉及到大米防御,其中OsSANRL.1-代谢的血清素显示出抗真菌活性.
结论:
- lncRNAs在调节对抗R. solani.米的防卫反应方面发挥着重要作用.
- 在植物病原体相互作用期间,lncRNA介导的cis调节和ceRNA网络对于代谢重编程至关重要.
- 特定的酶和它们的代谢产物,如血红素,直接促进了大米对囊病的免疫力.
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