Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

All-optical classification of real biomedical cell images using a diffractive neural network: a simulation study.

Optics letters·2026
Same author

Ultrafast Structural Dynamics of Biomolecular Complexes Probed by Broadband Time-Resolved Circular Dichroism.

The journal of physical chemistry letters·2026
Same author

Targeting fatty acid synthase suppresses tumor development in NF2/CDKN2A-deficient pleural mesothelioma.

Cell death & disease·2026
Same author

Chemotherapy resistance due to epithelial-to-mesenchymal transition is caused by abnormal lipid metabolic balance.

eLife·2026
Same author

Two-Photon-Excited Photoinduced Force Microscopy.

Nano letters·2025
Same author

Causal role of persistent neural activity in the mouse medial prefrontal cortex in promotion of wakefulness.

Neuroscience research·2025

相关实验视频

Updated: Sep 17, 2025

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy
09:49

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

Published on: October 8, 2013

16.8K

在小鼠大脑中无标签可视化状小根.

Yusuke Murakami1,2, Mutsuo Nuriya3,4, Zuliang Hu5

  • 1Ph.D. Program in Humanics, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan.

Analytical chemistry
|July 1, 2025
PubMed
概括

研究人员开发了一种新的成像方法来可视化Rootletin,这是神经元初级乳毛中的关键蛋白质. 这种技术有助于理解状功能和神经元发育.

更多相关视频

Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain
04:17

Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain

Published on: March 8, 2024

1.1K
Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex
09:55

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

8.5K

相关实验视频

Last Updated: Sep 17, 2025

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy
09:49

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

Published on: October 8, 2013

16.8K
Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain
04:17

Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain

Published on: March 8, 2024

1.1K
Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex
09:55

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

8.5K

科学领域:

  • 神经科学是一个神经科学.
  • 细胞生物学 细胞生物学
  • 生物物理学的生物物理.

背景情况:

  • 神经元初级毛对大脑发育和功能至关重要.
  • 根丁是纤维状根子必不可少的结构蛋白,但由于可视化挑战,其作用尚不清楚.

研究的目的:

  • 开发一种无标签,快速和灵敏的成像方法,用于在脑组织中可视化Rootletin.
  • 描述神经元中状根子的分布和动态.

主要方法:

  • 使用了多式多光子成像平台,将第二波代 (SHG) 显微镜与相干的反斯托克斯拉曼散射 (CARS) 结合起来.
  • 实施了一步化学预处理,以减少SHG成像中的背景.
  • 将平台应用于小鼠海马组织和培养的神经元.

主要成果:

  • 成功地可视化了神经元纤维根的高度组织和特定的细胞内分布.
  • 在成熟和不成熟的培养神经元中检测到状根.
  • 证明了该平台在神经科学研究中的敏感性和适用性.

结论:

  • 开发的无标签成像平台为研究状动态和神经元功能提供了强大的工具.
  • 提供了对神经元状根子结构组织的新见解.
  • 促进发展神经科学和神经退行性疾病的研究.