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在动脉样硬化中使用多组和单细胞分析识别与火死相关的基因和亚型

Kaisheng Jiang¹, Yang He¹, Bingjie Hu²

¹Faculty of Forensic Medicine, Guangdong Province Translational Forensic Medicine Engineering Technology Research Center, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, People's Republic of China.

Scientific reports

|July 1, 2025

概括

这项研究确定了与灭相关的基因 (PRG) 作为动脉样硬化 (AS) 炎症的关键驱动因素. TREM2被强调为一个关键的调节器,为AS诊断和治疗提供新的途径.

科学领域:

心血管生物学心血管生物学
免疫学免疫学免疫学
分子医学是分子医学.

背景情况:

动脉样硬化 (AS) 是一种由脂质代谢,免疫失调和细胞死亡驱动的慢性炎症性疾病.
炎症性编程细胞死亡的一种形式热,在AS中起作用,但其机制和治疗点尚未完全理解.

研究的目的:

在动脉样硬化中使用多组学方法识别和优先考虑与热致死相关的基因 (PRGs).
调查PRG在AS中的诊断潜力和治疗影响.

主要方法:

集成的多omics数据 (转录组学,单细胞RNA测序) 与机器学习.
进行功能丰富,免疫透和蛋白质-蛋白质相互作用网络分析.
使用体外和体外实验模型验证的结果.

主要成果:

确定了36个PRG,其中TREM2,TNF,MMP9,IL1B和CASP1被确定为关键调节剂.
在独立的数据集中,PRG显示出显著的诊断潜力.
发现了一种患者亚型 (集群2) 具有高度的巨细胞灭和促炎环境.
巨细胞/单细胞中的TREM2上调与免疫激活和斑块不稳定性相关,促进脂质积累和热.

结论:

与烧灭相关的基因,特别是TREM2,是AS进展的关键调节器.
这项研究为开发基于PRG的动脉样硬化的诊断和治疗策略提供了基础.

关键词:

动脉样硬化是一种动脉样硬化.免疫微环境是一个微环境.多个omics的分析分析.热灭菌的发生.在TREM2中,我们可以使用TREM2.

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