主导性和交叉反应性S1特异性B细胞记忆反应,由原发性SARS-CoV-2感染诱导
Xiaomin Xing1,2,3, Zhaoyong Zhang4, Yingfen Wen1,3
1Institute of Infectious Diseases, Guangzhou Eighth People's Hospital, Guangzhou Medical University, No.8 Huaying Road, Jiahe Street, Baiyun District, Guangzhou, 510440, China.
记忆B细胞 (MBCs) 主要针对SARS-CoV-2的S1域,与血清抗体不同. 针对SARS-CoV的交叉反应性MBC可能会提供对新变种的保护.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 传染性疾病 传染性疾病
背景情况:
- 与血清抗体相比,记忆B细胞 (MBCs) 在SARS-CoV-2免疫力中的作用尚不清楚.
- 了解MBC精细特异性对于疫苗开发和预测保护性免疫力至关重要.
研究的目的:
- 描述MBC对SARS-CoV-2的反应的特异性.
- 调查与SARS-CoV的交叉反应性和对变种的潜在保护.
主要方法:
- 使用一种无偏见的B细胞培养方法,每个井的MBCs有限.
- 具有针对SARS-CoV-2尖端蛋白域的MBC细特异性的特征.
- 来自S1结合的MBCs的单克隆抗体.
主要成果:
- 大血球细胞主要识别S1域,而血清抗体准S2.
- 显著频率的MBCs与SARS-CoV S1和RBD交叉反应.
- 衍生抗体对Omicron变异中和逃脱的抗性进行了证明.
结论:
- 主要的SARS-CoV-2感染会引起主导的S1指向的MBC反应.
- 针对SARS-CoV RBD的交叉反应性MBC可能会对新出现的SARS-CoV-2变种提供保护.
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