内细胞质精子注射 (ICSI) 时间影响精子DNA碎片化对ICSI结果的影响
Gaofeng Yan1, Dan Tang2, Zhenfang Su1
1Reproductive Genetics Department, The first affiliated hospital of Kunming Medical University, Kunming, 650032, China.
Reproductive sciences (Thousand Oaks, Calif.)
|July 2, 2025
概括
内细胞质精子注射 (ICSI) 时间影响活产率,特别是在高精子DNA碎片化 (SDF) 的男性中. 优化ICSI时间对于改善这些患者的治疗结果至关重要.
科学领域:
- 生殖医学 生殖医学
- 胚胎学 胚胎学
- 不孕症治疗 不孕症治疗
背景情况:
- 卵细胞DNA修复能力因卵细胞质量而异.
- 卵子采集后 (OPU) 长时间的潜伏可能导致卵子细胞衰老,影响超结构和功能.
- 精子DNA碎片化 (SDF) 可以影响细胞体内精子注射 (ICSI) 的结果.
研究的目的:
- 调查ICSI时间是否影响SDF对ICSI结果的影响.
- 根据OPU-ICSI时间间隔,对不同SDF级别之间的ICSI结果进行比较.
主要方法:
- 对1331个ICSI周期 (2016年3月 - 2022年12月) 的回顾性研究.
- 患者分为SDF<30%和SDF≥30%的组.
- 基于OPU-ICSI时间的三动物分析结果.
主要成果:
- 在SDF组之间,总体实验室或临床结果没有显著差异.
- 在最长的OPU-ICSI间隔 (第三次),SDF ≥30%组的临床怀孕率 (CPRs) 和活产率 (LBRs) 显著较低.
- 调整后的分析证实,高SDF组的CPRs (OR 0.425) 和LBRs (OR 0.485) 在第三层3.中较低.
结论:
- ICSI时间显著影响SDF对活产概率的影响.
- 对于高SDF水平的患者,确定最佳ICSI时间对于成功治疗结果至关重要.
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