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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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相关实验视频

Updated: Sep 17, 2025

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
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The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

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基于冷电子显微镜的内皮管映射

Ieva Drulyte1,2, Daniel L Hurdiss3

  • 1Materials and Structural Analysis, Thermo Fisher Scientific, Eindhoven, The Netherlands.

Methods in molecular biology (Clifton, N.J.)
|July 2, 2025
PubMed
概括
此摘要是机器生成的。

本章详细介绍了冷电子显微镜 (cryo-EM) 单粒子分析 (SPA) 协议,用于研究抗原-抗体相互作用. 它提供了检查这些复合物的提示,这些复合物对于药物和疫苗开发至关重要.

关键词:
抗体是一种抗体.低温电子显微镜的使用方法在地图上绘制出地图的地图.皮托普-帕拉托普 - 皮托普-帕拉托普蛋白质与蛋白质之间的相互作用一个粒子分析分析.结构生物学是结构生物学.

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科学领域:

  • 结构生物学 结构生物学
  • 免疫学 免疫学 免疫学
  • 生物化学 生物化学

背景情况:

  • 抗体是适应性免疫系统的关键组成部分.
  • 抗体-抗原相互作用对于治疗,疫苗和理解自我/非自我认知至关重要.
  • 低温电子显微镜单粒子分析 (cryo-EM SPA) 是研究分子相互作用的强大工具.

研究的目的:

  • 为使用冷EM SPA检查抗原-抗体复合物的标准化方案提供.
  • 为成功的冷EM SPA提供这些相互作用的实用技巧和技巧.
  • 促进治疗性抗体开发和免疫学研究的研究.

主要方法:

  • 低温电子显微镜单颗粒分析 (cryo-EM SPA).
  • 针对抗原-抗体复合成像的详细协议开发.
  • 数据采集和处理的优化策略.

主要成果:

  • 对抗原-抗体复合体的冷-EM SPA 进行全面和可适应的协议.
  • 确定方法论中的关键参数和潜在陷.
  • 证明冷-EM SPA的实用性,以阐明这些相互作用的结构.

结论:

  • 化EM SPA是一种高分辨率的有效方法来表征抗原-抗体复合物.
  • 提供的协议和提示可以加速基于抗体的治疗和诊断研究.
  • 这项工作增强了冷EM在结构免疫学和药物发现中的应用.