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Updated: Sep 17, 2025

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
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使用细菌细胞表面显示的基因片段图书馆对抗体的皮质映射

Anna-Luisa Volk1, Francis Jingxin Hu1, Magdalena Malm1

  • 1Department of Protein Science, KTH-Royal Institute of Technology, Stockholm, Sweden.

Methods in molecular biology (Clifton, N.J.)
|July 2, 2025
PubMed
概括

我们开发了一种高分辨率的方法,使用细菌表达和流细胞计绘制抗体结合部位 (表位) 的地图. 这种技术精确地识别了标蛋白的表位,提高了测试中的抗体可靠性.

关键词:
抗体是对抗体的一种.细胞表面显示器显示器在地图上绘制出地图的地图.传真 (FACS) 是一个事实.格拉姆阳性菌种.这种动物叫S. carnosususus.

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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