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Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
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通过高密度竞争ELISAs绘制B细胞内皮质映射

David J Vance1, Nicholas J Mantis2

  • 1Division of Infectious Disease, New York State Department of Health, Wadsworth Center, Albany, NY, USA. David.Vance@health.ny.gov.

Methods in molecular biology (Clifton, N.J.)
|July 2, 2025
PubMed
概括

竞争ELISAs快速对抗蛋白抗原的单克隆 (MAb) 和单域 (VHH) 抗体. 这种方法为资源密集的表位图绘制技术提供了有效的替代方案.

关键词:
抗体是一种抗体.竞争 竞争 竞争 竞争 竞争艾丽莎·伊丽莎·伊丽莎上层层是什么意思 上层是什么意思纳米体是一种纳米体.

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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