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Updated: Jun 26, 2026

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging
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在自由移动的动物中,闭环双光子功能成像

Paul McNulty1, Rui Wu1, Akihiro Yamaguchi1

  • 1Department of Physics, New York University, New York, NY, USA.

Nature communications
|July 2, 2025
PubMed
概括

我们开发了一种新的高速双光子显微镜,以准确测量自由运动的动物的神经活动. 这项技术克服了运动器件,使大脑功能和行为的新发现成为可能.

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Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...