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Updated: Sep 17, 2025

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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在产品发布后,多次周转Cas9的可视化

Kaitlyn A Kiernan1,2, David W Taylor3,4,5,6

  • 1Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA.

Nature communications
|July 2, 2025
PubMed
概括

对于CRISPR基因编辑至关重要的Cas9酶,在切割后经常与DNA结合,阻碍修复. 这项研究揭示了目标部位附近的DNA片段可以防止Cas9脱离,从而提高基因编辑效率.

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相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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