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相关概念视频

Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.6K

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相关实验视频

Updated: Sep 17, 2025

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping
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High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping

Published on: March 22, 2024

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在高分辨率的DNA结构中量化复杂性 原子力显微镜

Elizabeth P Holmes1, Max C Gamill1, James I Provan2,3

  • 1School of Chemical, Materials and Biological Engineering, University of Sheffield, Sheffield, UK.

Nature communications
|July 2, 2025
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种新的自动化管道,将原子力显微镜 (AFM) 和深度学习结合起来,以分析DNA拓. 这种方法用高分辨率量化DNA的长度,形状和复杂的拓结构.

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Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
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科学领域:

  • 分子生物学分子生物学
  • 生物物理学的生物物理.
  • 基因组学就是基因组学.

背景情况:

  • DNA拓对于细胞功能和基因组稳定性至关重要.
  • 在复杂分子中量化DNA拓是技术上具有挑战性的.
  • 现有的方法缺乏详细分析的吞吐量和分辨率.

研究的目的:

  • 开发一种高通量,高分辨率的方法来量化DNA拓.
  • 分析复杂DNA分子的结构和拓,包括复制中间体和重组产物.
  • 为了研究表面固定对DNA拓学的影响.

主要方法:

  • 高分辨率的原子力显微镜 (AFM) 结合了一种新的自动化深度学习管道.
  • 深度学习用于DNA骨干跟踪和识别交叉点.
  • 粗粒度模拟用于模拟表面固定效应.

主要成果:

  • 该管道准确量化了个别DNA分子的长度,形状和拓.
  • 分析停滞的复制中间体 (甲基结构,晚期产物) 和大肠杆菌XER重组产物 (等离子体,结,连锁体).
  • 测量表面固定效应对扭转式分区的量化.

结论:

  • 开发的管道为DNA拓分析提供了前所未有的分辨率和吞吐量.
  • 这种方法可以对生物学上相关的DNA分子进行详细的结构和拓研究.
  • 这些发现提供了关于DNA拓如何调节基本生物过程的见解.