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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: Sep 17, 2025

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

用多重超分辨率光显微镜绘制核景观的地图.

Fariha Rahman¹, Victoria Augoustides², Emma Tyler¹

¹Lampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, North Carolina State University, Chapel Hill, NC, USA.

Nature communications

|July 2, 2025

概括

这项研究可视化了使用新型成像协议的核组织. 它揭示了活跃和压制性的核国家如何从微观到纳米规模组织起来.

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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Published on: December 9, 2013

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

相关实验视频

Last Updated: Sep 17, 2025

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

细胞核协调复杂的生物过程.
了解DNA,蛋白质和表观遗传标记的空间组织至关重要.
现有的成像技术在解决纳米组织方面存在局限性.

研究的目的:

开发一个多重成像协议,用于核目标的高精度定位.
为了研究核的空间组织跨不同的长度尺度.
揭示积极和压制性的核国家组织原则.

主要方法:

开发了一种用于纳米精度定位的多重成像协议.
在单个细胞内分析了13个核目标的空间相关性.
研究了扰乱质子乙化和转录对核组织的影响.

主要成果:

核规范到活性/抑制状态跨越多个长度尺度,在纳米尺度加强.
异色素和欧色素表现出独特的纳米级组织原理.
基质子乙化或转录扰乱破坏了纳米级组织,但不是微观组织.

结论:

开发的成像和分析管道为核组织原理提供了洞察力.
纳米级组织在定义活跃和压制性的核国家方面发挥着关键作用.
这种方法可以应用于研究其他细胞区域.