全球病原体呼吸道合成病毒和人类诺罗病毒的完整基因组表征,采用探针式捕获丰富方法
Sravya V Bhamidipati1, Anil Surathu2,3, Hsu Chao1
1Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX, 77030, USA.
Scientific reports
|July 2, 2025
概括
新的寡核酸探针集显著改善了呼吸道同胞病毒 (RSV) 和人类诺罗病毒 (HuNoV) 的全长基因组测序. 这一进步有助于追踪这些重要的人类病原体的病毒多样性和新兴变异.
科学领域:
- 病毒学 病毒学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 呼吸道同胞病毒 (RSV) 和人类诺罗病毒 (HuNoV) 分别是导致呼吸道和胃肠道疾病的主要原因.
- 了解病毒多样性和跟踪变异需要高质量的全长基因组序列.
- 获取此类数据往往受到病毒菌株变异性,质量和低位数的阻碍.
研究的目的:
- 开发和验证用于增强RSV和HuNoV基因组的捕获和测序的新型寡核酸探针集.
- 提高从临床样本中完全恢复病毒基因组的效率和成功率.
主要方法:
- 基核酸探针集的设计基于来自GenBank的1570个RSV和1376个HuNoV序列.
- 对85个RSV和55个HuNoV阳性临床样本应用了捕获丰富测序工作流.
- 测序数据分析了病毒读取映射百分比和基因组恢复率,捕获前后.
主要成果:
- 捕获后的测序显示病毒读取映射百分比明显更高:RSV为85.1%,HuNoV为40.8%,与捕获前 (0.08%和1.15%) 相比.
- 从所有RSV阳性样本和55个HuNoV阳性样本中47个中成功恢复了全长基因组.
- 这代表了基因组恢复效率的大幅提高.
结论:
- 开发的寡核酸探针集和捕获丰富工作流显著提高了全长RSV和HuNoV基因组的恢复.
- 这种方法为基因组监测,变种跟踪和理解这些关键人类病毒的多样性提供了一个强大的工具.
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