DDTC-Cu(I) 通过抑制MET/PI3K/AKT信号通路来抑制人类骨髓瘤细胞的生长
Ruhao Zhou1,2, Lei Yan1,2, Kun Zhang1,2
1Second Clinical Medical College, Shanxi Medical University, 382 Wuyi Road, Taiyuan, 030001, Shanxi, People's Republic of China.
Scientific reports
|July 2, 2025
概括
迪苏尔菲拉姆-铜 (DDTC-Cu(I)) 通过诱导亡并抑制癌细胞生长,迁移和入侵,有效地对抗骨髓瘤 (OS). 这种新的治疗方法对治疗这种侵袭性骨癌具有极小副作用的前景.
科学领域:
- 在瘤学瘤学.
- 药理学 药理学是指药理学的学科.
- 生物化学 生物化学
背景情况:
- 骨髓瘤 (OS) 是一种具有高转移潜力和死亡率的原发性骨癌.
- 用于治疗酒精依赖的迪苏尔菲拉姆 (DSF) 在体内形成DDTC-Cu,表现出抗瘤特性.
研究的目的:
- 在体外和体内研究DDTC-Cu(I) 对OS细胞的疗效.
- 阐明潜在的分子机制,重点关注MET和PI3K/AKT信号通路.
主要方法:
- 在体外研究中使用了OS细胞系 (U2OS,SaOS2,MG-63) 的分析,包括CCK-8,殖民地形成,RTCA,穿孔入侵,流细胞计,伤口愈合和RNA测序.
- 在体内,使用SaOS2异种移植小鼠模型和主要器官组织学分析证实了疗效.
主要成果:
- DDTC-Cu ((I) 剂量取决于诱导的亡和抑制OS细胞中的增殖,迁移和入侵.
- 该药物向MET和PI3K/AKT信号通路;MET过度表达部分逆转了DDTC-Cu的作用.
- 在体内研究证实了DDTC-Cu的通过MET抑制的抗OS疗效,但对主要器官没有显著的不良影响.
结论:
- DDTC-Cu(I) 显示出作为抗骨髓瘤治疗剂的显著潜力.
- 针对MET和PI3K/AKT通路对于DDTC-Cu的抗OS活性至关重要.
- DDTC-Cu(I) 是一个有前途的,安全的治疗选择,用于骨髓瘤治疗.
相关概念视频
mTOR Signaling and Cancer Progression
3.9K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.9K
PI3K/mTOR/AKT Signaling Pathway
4.0K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
4.0K
Inhibition of Cdk Activity
4.9K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.9K
Drugs that Stabilize Microtubules
2.1K
Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
2.1K


