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单细胞转录组学分析显示,肝转移性癌症中NK-T细胞相互作用网络中断

Xiaoshuang Wang¹, Zhongen Wu², Yan Zhou³

¹Shanghai Cancer Center, Shanghai Medical College, Fudan University, Shanghai, 200032, China.

Scientific reports

|July 2, 2025

概括

这项研究揭示了包括CD8+NKT类细胞在内的特定T细胞类型如何激活自然杀手 (NK) 细胞来对抗肝脏转移. 这种NK-T细胞相互作用为癌症提供了潜在的新治疗点.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
系统生物学系统生物学

背景情况:

自然杀手 (NK) 细胞和T细胞对于通过瘤细胞细胞毒性控制转移性肝癌至关重要.
肝转移中NK和T细胞之间的精确相互作用网络尚不清楚.
了解这种网络是开发新型癌症疗法的关键.

研究的目的:

为了研究NK-T细胞相互作用网络在不同癌症类型的肝转移中.
阐明这种相互作用对抗瘤活性的功能影响.
在NK-T细胞轴内识别潜在的治疗点.

主要方法:

从鼻癌,甲状腺癌,乳腺癌,结肠直肠癌和宫癌中分析肝转移和邻近组织的单细胞转录学分析.
免疫细胞透的分析,专注于NK细胞,T细胞和NKT类细胞.
研究细胞间的通讯路径和信号布.

主要成果:

在相邻组织中观察到CD8+NKT样细胞,γδT细胞和NK细胞的透增加.
增强了CD8+NKT样细胞,CD8+T细胞,γδT细胞和NK细胞之间的相互作用,与T和NK细胞功能相关联.
发现CD8+NKT样细胞,CD8+T细胞和γδT细胞通过CD48-CD244和TNF-TNFR通路激活NK细胞,激活ERK,JNK和MAPK级联.

结论:

在肝转移性癌症中发现了详细的NK-T细胞相互作用网络.
特定的T细胞子集通过定义的信号通路增强NK细胞的抗瘤活性.
已确定的NK-T细胞相互作用代表了肝转移性癌症的有希望的治疗途径.

关键词:

肝脏转移的发生.NK-T 相互作用的相互作用单细胞转录组学单细胞转录组学

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