利用微藻中的外聚糖来阻止Bothrops sp.体外有毒作用. 毒药是一种毒药
Camila Castro-Pinheiro1, Stanislav Sukhikh2, Olga Babich2
1Fluminense Federal University, Niteroi, Rio de Janeiro, Brazil.
Scientific reports
|July 2, 2025
概括
微藻外聚糖体显示出作为蛇毒的补充疗法的潜力,抑制毒液.
科学领域:
- 海洋生物学 海洋生物学
- 生物化学 生物化学
- 毒理学 毒理学 毒理学
背景情况:
- 毒蛇咬伤会导致严重的局部组织损伤和全身毒性,目前的抗毒药物往往无法充分解决这一问题.
- 需要补充疗法来管理蛇毒的残留局部影响,如肌肉损伤和凝血病.
研究的目的:
- 评估来自四种微藻类的外聚糖类对Bothrops蛇毒的关键酶活性的抑制潜力.
- 评估这些微藻外聚糖的安全性,特别是它们的血液毒性潜力.
主要方法:
- 外聚糖是从Chlorella sorokiniana,Scenedesmus obliquus,Nannochloris sp.中提取出来的. 诺曼,以及一个叫做Scenedesmus acuminatus.
- 进行了体外试验测试,以测量Bothrops jararaca,B. jararacussu和B. neuwiedi毒素的蛋白质分解,血凝固剂和脂酶A2 (PLA2) 活动的抑制.
- 血毒性通过评估外聚糖在红细胞上的溶血活性来评估.
主要成果:
- 来自S. obliquus和Nannochloris sp.的外聚糖类. 纳曼证明了毒素蛋白质活性的显著抑制 (分别高达99%和97%).
- 这些外聚糖还防止了毒素诱导的血凝结,并降低了PLA2活性 (分别为15%和30%).
- 测试的微藻外聚糖都没有表现出血解活性,这表明它们是非血毒的.
结论:
- 微藻衍生的外聚糖类对关键有毒酶具有显著的毒素抑制特性.
- 这些化合物表现出良好的安全性,缺乏血毒性.
- 来自诸如S. obliquus和Nannochloris sp.等物种的外聚糖. 纳曼是开发作为蛇毒的补充治疗方法的有希望的候选人.
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