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Updated: Sep 17, 2025

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
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引入多重二硫化键增加了转胺酶的热稳定性

Takuto Ono1, Kazutoshi Takahashi2, Yoshinori Hirao1

  • 1Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki-shi, Kanagawa-ken, 210-8681, Japan.

Scientific reports
|July 2, 2025
PubMed
概括

将二硫化键添加到微生物转胺酶 (MTG) 中,显著提高了其热稳定性. 这一策略通过加强关键蛋白质区域来提高工业应用的酶性能.

关键词:
双硫化物键是一种二硫化物键.酶动力学 酶动力学微生物的转谷氨酸酶.一个突变的突变者.热稳定性 热稳定性

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