使用分子条形码和全基因组测序来探索来自木麻根球的Bacillus subtilis分离物的遗传多样性和基因组洞察力
Supajit Sraphet1, Bagher Javadi2
1Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, 73170, Thailand.
Scientific reports
|July 2, 2025
概括
这项研究使用全基因组测序来分析来自麻土的Bacillus subtilis菌株. 重复的DNA序列分化了菌株,揭示了生物肥料等农业应用的遗传多样性.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 农业科学 农业科学
背景情况:
- 细菌在农业和工业中至关重要,通常在土壤中发现.
- 了解B. subtilis的遗传多样性是优化其有益作用的关键.
研究的目的:
- 为了进行DNA条形编码和B. subtilis从木麻根球的全基因组测序.
- 分析遗传多样性并确定B. subtilis菌株的有益特征.
主要方法:
- 16S rDNA测序用于初始物种识别.
- 使用Illumina MiSeq.进行全基因组测序.
- 用于基因组指纹的重复性DNA元素 (REP和BOX序列) 的分析.
主要成果:
- 16S rDNA 在菌株之间显示出高度相似性 (99.98%).
- 重复的DNA序列提供了清晰的基因组指纹,并揭示了>97%的家族遗传距离.
- 对BsPr8菌株的全基因组测序产生了56个结合基因,3575个注释蛋白质和45个抗生素耐药基因.
结论:
- 与16S rDNA相比,全基因组分析提供了优越的菌株分化.
- 已识别的B. subtilis菌株具有有利于可持续农业的特征,包括促进植物生长和抑制病原体.
- 这些发现支持B. subtilis作为生物肥料和生物杀虫剂用于麻种植的发展.
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