相关实验视频

Updated: Jun 20, 2026

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 Kir4.1

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 Kir4.1

Published on: September 26, 2015

综合性表观基因组和转录基因组分析揭示了小细胞肺癌中ASCL1和NEUROD1不同调节的转录程序

Hiroshi Takumida¹, Akira Saito¹, Yugo Okabe^1,2

¹Department of Respiratory Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, 113-0033, Japan.

|July 2, 2025

概括

小细胞肺癌 (SCLC) 的可塑性在近一半的病例中涉及ASCL1和NEUROD1的共同表达. 这些因素调节了不同的基因,创造了中间亚型,为这种侵略性癌症提供了新的治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

小细胞肺癌 (SCLC) 是一种具有不良预后的侵袭性神经内分泌癌.
ASCL1和NEUROD1是SCLC中神经内分泌分化的关键调节者.
尚不清楚SCLC的可塑性,可能涉及ASCL1-阳性 (SCLC-A) 和NEUROD1-阳性 (SCLC-N) 亚型之间的过渡.

研究的目的:

阐明SCLC中ASCL1和NEUROD1调节的转录程序.
识别与SCLC可塑性相关的分子标记物.
研究ASCL1/NEUROD1共同表达在SCLC异质性中的作用.

主要方法:

免疫组织化学评估ASCL1和NEUROD1蛋白质表达.
对基因组修饰和转录因子结合位点 (ASCL1,NEUROD1) 的整个表观基因组的分析.
在SCLC细胞中进行全转录组分析 (RNA-seq,小RNA-seq),包括NEUROD1淘汰模型.

主要成果:

在近一半的SCLC病例中发现了ASCL1和NEUROD1共同表达 (SCLC-A/N).
这两种因素都在SCLC-A/N中转录活跃,调节不同的基因.
SCLC-A/N亚型表现出SCLC-A和SCLC-N之间的中间特征.
NEUROD1的淘汰表明与NHLH转录因子的联系.
特别确定了miR-139-5p在NEUROD1-阳性SCLC中,可能与其他miRNA共同调节目标基因.

结论:

ASCL1和NEUROD1在控制SCLC转录程序方面发挥着重要的,部分不同的作用.
ASCL1和NEUROD1的共同表达有助于SCLC异质性和中间表型.
综合性分析揭示了多层监管网络,包括miRNA参与,为SCLC可塑性提供了洞察力.

更多相关视频

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

相关概念视频

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...

Somatic to iPS Cell Reprogramming

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...