遗传变异与西非Spodoptera frugiperda (Lepidoptera: Noctuidae) 的lambda-cyhalothrin耐药性增加有关
Eric Tossou1,2, Ghislain T Tepa-Yotto3,4, Georg Goergen3
1Agroecohealth Unit , International Institute of Tropical Agriculture (IITA) , 08 P.O. Tri-Postal, Box 0932, Cotonou, Benin. eric.tossou82@gmail.com.
Scientific reports
|July 2, 2025
概括
西非的秋季军对常见的杀虫剂表现出中度的敏感性. 抵抗机制涉及代谢酶和点突变,这对于可持续的害虫控制策略至关重要.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 虫害管理 虫害管理 虫害管理
- 分子生物学分子生物学
背景情况:
- 秋季军虫 (Spodoptera frugiperda) 是一种破坏性的入侵性害虫,影响着全球农业,特别是玉米作物.
- 了解杀虫剂易感性和耐药机制对于有效的控制计划至关重要.
研究的目的:
- 为了描述西非秋季军虫种群的特征.
- 评估它们对关键杀虫剂 (λ-cyhalothrin, chlorpyrifos, emamectin benzoate) 的敏感性.
- 调查潜在的抵抗机制,包括点突变和生化途径.
主要方法:
- 使用RFLP-PCR和TPI测序进行种群表征.
- 昆虫杀虫剂敏感性的生物测试.
- 协同作用测试用于评估代谢酶作用.
- 定量PCR (qPCR) 用于目标突变分析.
主要成果:
- 主要确定了玉米菌株种群 (72.5-100%).
- 对于 λ-cyhalothrin,pyrifos 和 emamectin benzoate 观察到易受中度毒性.
- 代谢酶 (氧化酶,雌激酶,GST) 在l-cyhalothrin耐药性中起到中等作用.
- 与生物化学机制和特定突变 (F290V,T929I) 相关的对有机酸盐和铁酸盐的耐药性.
结论:
- 提供了关于秋季军虫在西非抗虫剂耐药性的关键数据.
- 为开发可持续的害虫管理和耐药性管理策略提供信息.
- 强调生物化学机制和点位突变在抗昆虫剂耐药性的联合作用.
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