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通过AlSE,通过肠道微生物利用替代甜味剂D-糖
Glory Minabou Ndjite1, Angela K Jiang1,2, Charlotte T Ravel1
1College of Computer, Mathematical and Natural Sciences, University of Maryland, College Park, MD, USA.
Communications biology
|July 2, 2025
概括
D-粉是一种有前途的低热量甜味剂. 研究表明,肠道微生物组的代谢能力有限,这表明胃肠道问题最小,并有可能进行个性化的营养策略.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 营养科学 营养科学
背景情况:
- D-粉是一种罕见的糖甜剂,具有低热量特性,可以替代导致胃肠道疾病的甜味剂.
- D-氨酸-6-酸3-胺酶 (AlsE) 是一种参与D-氨酸代谢的酶,但其在肠道细菌中的患病率在很大程度上是未知的.
研究的目的:
- 研究Alse在细菌D-粉代谢中的分类学分布和功能作用.
- 评估Alse在人类肠道微生物群中的患病率及其对D-allulose利用的影响.
主要方法:
- 细菌基因组的生物信息分析,以确定 AlsE 同类物.
- 在Clostridium innocuum和大肠杆菌中对D-粉素代谢的实验验证.
- 对人类肠道样本进行元基因组分析,以确定SE的流行率.
主要成果:
- 在116种细菌物种中发现了Alse同类物种,表明分布有限.
- 克洛斯特里无害菌通过其同类AlSE通过D-allulose进行代谢.
- 在大肠杆菌中alsE的异质表达使得D-allulose的利用成为可能.
- 在分析的人类肠道转基因组的15.8%中检测到alsE.
结论:
- 人的肠道微生物组对通过AlSE的D-氨酸代谢能力有限.
- 由于D-allulose的代谢有限,因此可以将其作为一种甜味剂,对肠道微生物群和胃肠道症状的影响最小.
- 这些发现使得个性化的营养方法能够管理血糖反应并减少消化不适.
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