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Updated: Sep 17, 2025

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox
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Published on: July 5, 2021

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基于扩散的单分子FRET实验的无模型光子分析.

Ivan Terterov1, Daniel Nettels2, Tanya Lastiza-Male3,4

  • 1Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot, Israel. ivan.terterov@weizmann.ac.il.

Nature communications
|July 2, 2025
PubMed
概括

通过分析单分子弗斯特共振能量转移 (smFRET) 数据,本研究引入了一种无模型的方法. 它准确地量化了各种时间尺度上的蛋白质动态,验证了当前的分析技术.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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