在人类慢性感染期间,Yersinia enterocolitica在宿主中的进化
Cyril Savin1,2,3, Pierre Lê-Bury4,5,6, Julien Guglielmini7
1Institut Pasteur, Université Paris Cité, Yersinia Research Unit, Paris, France. cyril.savin@pasteur.fr.
Nature communications
|July 2, 2025
概括
Yersinia enterocolitica在一个病人身上在14年内进化,失去基因并获得抗生素耐药性. 这种细菌适应涉及基因组缩小和改变蛋白质生产,影响治疗结果.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 进化生物学 进化生物学
背景情况:
- 细菌对抗生素治疗等环境压力表现出显著的适应能力.
- 病原细菌可以在长期感染期间在宿主中演变.
研究的目的:
- 在14年的慢性感染期间,描述Yersinia enterocolitica的遗传和表型演变.
- 为了确定与抗生素耐药性和宿主适应相关的特定遗传突变和蛋白质变化.
主要方法:
- 序列细菌分离物的全基因组测序.
- 蛋白质组分析以评估蛋白质表达和重塑.
- 对抗生素敏感性和生长特征进行表型检测.
主要成果:
- 确定了基因组的减少 (大约. 100个基因丢失) 和 gyrA 中的一种新型缺失,赋予了诺隆耐药性.
- 观察到与OmpF截断和增高β-乳糖酶 (BlaA, AmpC) 生产相关的第三代脑素耐药性.
- 检测到显著的蛋白质组重塑,包括扰乱严格反应和代谢受损,导致体外生长缺陷.
结论:
- 在抗生素压力下的长期宿主进化过程中,Yersinia enterocolitica经历了大量的遗传和表型变化.
- 这些适应性有助于抗生素耐受性,并可能解释治疗失败.
- 这项研究突出了以前未经记录的在宿主环境中的细菌适应机制.
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