开发SPE-LC-MS/MS方法用于确定WHO AWaRe 医院废水中的储备抗生素
Joanna Wilk1, Paulina Sowik2, Ewa Felis2
1Department of Inorganic Chemistry, Analytical Chemistry, and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Krzywoustego 6 Str., 44-100, Gliwice, Poland.
Scientific reports
|July 2, 2025
概括
这项研究开发了一种检测医院废水中的关键储备抗生素的方法,在所有采样的波兰医院中发现了它们. 早期环境监测对于打击抗菌素耐药性至关重要.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 公共卫生 公共卫生
背景情况:
- 抗菌素耐药性 (AMR) 是一种全球性健康威胁,其原因是过度使用抗生素和监管不足.
- 世界卫生组织的AWaRe分类有助于谨慎使用抗生素,强调了储备抗生素的关键作用.
- 医院的废水是传播抗菌素耐药性基因和耐药细菌的潜在储存库.
研究的目的:
- 开发和验证一种用于确定医院废水中的储备抗生素的方法.
- 在医院的废水中量化特定的储备抗生素 (阿兹特洛纳姆,线索利德,梅罗,蒂吉赛克林,瓦博巴克坦,基拉斯塔丁,斯福米辛,塞夫塔齐迪姆) 的存在.
- 建立波兰高优先级抗生素环境监测的基准.
主要方法:
- 优化26个提取程序,以实现目标储备抗生素的最佳恢复率 (31.5-103.7%).
- 使用液体染色学-并联质谱法 (LC-MS/MS) 分析抗生素残留物.
- 验证开发的分析方法的准确性和精度.
主要成果:
- 在波兰所有16家采样医院的废水中检测到储备抗生素.
- 检测到的储备抗生素度在1.89 ng L-1至22.49 μg L-1.1之间.
- 这项研究是首次在全国范围内对医院废水中的储备抗生素进行方法调查.
结论:
- 开发的LC-MS/MS方法对于监测医院废水中的储备抗生素是有效的.
- 这些关键抗生素在医院污水中无处不在的存在强调了对环境监测的必要性.
- 将废水监测纳入医疗保健战略可以帮助缓解全球抗菌素耐药性危机.
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