通过FTIR光谱学开发可转移和通用的红外线生物标志物,用于手术内结直肠癌的诊断
Ran Li1, Heather C Allen2,3
1Department of Chemistry and Biochemistry, The Ohio State University, 100 West 18th Avenue, Columbus, OH, 43210, USA.
Scientific reports
|July 2, 2025
概括
里埃变换红外光谱法 (FTIR) 显示出对客观癌症检测的希望. 一个关键的生物标志物 (b1) 在分化结直肠组织方面表现出强度,这表明了手术内应用的潜力.
科学领域:
- 生物医学光谱学 生物医学光谱学
- 癌症的诊断 癌症的诊断
- 机器学习在医学中的应用
背景情况:
- 组织学染色,目前的癌症检测标准,遭受主观性和延迟.
- 福里埃变换红外光谱法 (FTIR) 提供客观的实时分析,但由于不同的实验条件,生物标志物的概括性面临挑战.
- 开发可转移的IR生物标志物对于癌症诊断中的临床转化至关重要.
研究的目的:
- 开发和评估强大的IR生物标志物,以区分结直肠癌组织.
- 评估基于FTIR的生物标志物在不同实验条件下的通用性和可转移性.
- 建立一个推进非侵入性红外光谱在手术内癌症检测的基准.
主要方法:
- 从结直肠癌病例中系统地收集组织光谱.
- 使用已识别的光谱带比率开发IR生物标志物.
- 使用机器学习对组织分化 (瘤,正常,边缘) 的生物标志物性能评估.
主要成果:
- 确定了三个主要的生物标志物 (b1: 1740/1236,b2: 1740/1162,b3: 1740/1080).这些生物标志物包括:
- 生物标志物b1在一个新病例中显示出瘤,正常和边缘组织的强有力的分化.
- 生物标志物b2和b3表现不那么令人满意,表明对异质性或实验变异的敏感性.
- 结合2-3个生物标志物增强了组织分化,在添加更多生物标志物时,回报会减少.
结论:
- 红外生物标志物b1在结直肠癌检测中表现出卓越的稳定性和对分子特征的敏感性.
- 基于FTIR的生物标志物需要仔细验证,以克服由组织异质性和实验变异性所造成的限制.
- 这项研究为开发可靠,非侵入性FTIR方法的基础,用于手术内癌症边际评估.
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