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一种反应性中间除氨酶A同类的生物化学和结构特征,来自Streptococcus sanguinis

Alexa B Benedict^1,2, Adreana I Aquino¹, Brandi A Buckner³

¹Department of Biological and Biomedical Sciences, Southwestern Oklahoma State University, Weatherford, OK, USA.

Scientific reports

|July 2, 2025

概括

血杆菌RidA (SsRidA) 清除了代谢压力因子2-氨基酸酸 (2AA) 的毒素. 这种酶将2AA去胺化为pyruvate,保护细胞免受损伤,并恢复突变细菌的生长.

科学领域:

生物化学生物化学
酶学是一种酶学.
微生物的新陈代谢

背景情况:

2-氨基酸盐 (2AA) 是一种反应性中间体和代谢性压力.
反应性中间除氨酶 (Rid) 蛋白质在所有生命领域中排毒2AA.
SsRidA是一种新发现的RidA同源,来自Streptococcus sanguinis.

研究的目的:

描述SsRidA的酶活性和结构性质.
为了确定SsRidA是否可以排毒2AA并保护细胞免受其影响.
为了阐明SsRidA的催化机制.

主要方法:

酶分析 (2AA 脱氨酶,L-氨基酸氧化酶).
在Salmonella enterica ridA突变体中的功能补充.
用于结构分析的X射线晶体学.

主要成果:

SsRidA表现出2AA除氨酶的活性,将2AA转化为pyruvate.
SsRidA显示L-氨基酸氧化酶活性对抗伊米诺-氨基酸.
在2AA压力下,SsRidA恢复了S. enterica ridA突变体的生长.
晶体结构显示出一个具有在单体接口上的活性位点的同位素蛋白.

结论:

SsRidA是一种功能性的2AA除氨酶和RidA同源.
结构表明一种触媒机制,涉及活性部位氨酸和水.
在S. sanguinis. 中,SsRidA在减轻2AA诱导的代谢压力方面发挥着至关重要的作用.

关键词:

血型链球菌 (Streptococcus sanguinis) 是一种血型链球菌.2 - 氨基烯酸二烯酸二烯酸二氨基烯酸二烯酸二氨基烯酸二氨基烯酸二氨基烯酸二氨基烯酸二氨基烯酸二氨基烯酸.胺去氨基酶是什么?代谢性压力是一种代谢性压力.骑士骑士是一个

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