相关实验视频

Updated: Sep 17, 2025

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi

Published on: June 6, 2025

通过固态发酵,从廉价农业废物中生物资源采购和优化真菌脂酶的生产

Gboyega E Adebami¹, Bukola C Adebayo-Tayo², Arindam Kuila³

¹Department of Biological Sciences, Mountain Top University, Ibafo, Nigeria. gboyega.adebami@gmail.com.

Scientific reports

|July 2, 2025

概括

研究人员确定了Aspergillus oryzae GP11,这种真菌能够使用廉价的农业废物基质,如小麦和大米,产生大量的脂酶. 这一发现为微生物脂酶生产提供了一种具有成本效益的方法.

科学领域:

微生物学微生物学
生物技术是生物技术.
酶学是一种酶学.

背景情况:

经济上可行的微生物脂酶来源对于工业应用至关重要.
农业废物是微生物发酵中未充分利用的资源.

研究的目的:

使用成本效益高的农业废物基质,从真菌中选,识别和产生脂酶.
通过介质组成和农业废物选择,优化脂酶生产.
为了描述纯化的脂酶.

主要方法:

从棕油厂的废水中选脂质真菌,使用固体和浸泡发酵.
使用形态和分子技术识别真菌分离物.
用p-nitrophenyl palmitate (p-NPP) 来测量脂酶活性的光谱测定.
固态发酵 (SSF) 使用各种农业废物和优化生产介质.
使用Sephadex G-100染色学进行脂酶净化.

主要成果:

阿斯珀吉勒斯 (GP11) 呈现出最高的脂酶活性 (加入编号:MN416218).
中等成分显著影响了脂酶和生物质生产,葡萄糖和产生最佳结果.
在测试的农业废物中,小麦 (230.78 U/mL) 和大米 (211.62 U/mL) 的脂酶产量最高.
净化脂酶的分子量为53kDa,具有显著的净化和产量.

结论:

Aspergillus oryzae是微生物脂酶生产的一个有希望的来源.
农业废物,特别是小麦和大米,是经济高效的脂酶发酵的有效基质.
描述的脂酶具有各种工业应用的潜力.

关键词:

农业废弃物 - 农业废弃物菌脂酶是一种真菌脂酶.脂质酶净化净化方法中等成分的组成.矿物质组成矿物质组成大致的组成.固态发酵发酵过程中的固态发酵.

更多相关视频

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution

Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution

Published on: December 30, 2021

相关概念视频

Lipid Catabolism

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...

Environmental Applications of Microorganisms

Environmental Applications of Microorganisms

Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...

Overview of Fungi

Overview of Fungi

Fungi are a diverse group of eukaryotes more closely related to animals than other eukaryotes. Fungal cell walls comprise chitin, a polysaccharide that provides structural strength, and glucans, which contribute to flexibility and integrity. Other polysaccharides, such as mannans and galactosans, may supplement or replace chitin in some fungi. These adaptations, along with their preference for acidic environments and tolerance for high osmotic pressure, enable fungi to thrive in various...