在微环境中长期居住的脂肪衍生干细胞重塑BLCA细胞干细胞和EMT促进膀癌的进展

在微环境中长期居住的脂肪衍生干细胞重塑BLCA细胞干细胞和EMT促进膀癌的进展

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Denggao Huang^1,2, Na Guo^1,2,3, Yanling Peng^1,2,4

¹Central Laboratory, Haikou Affiliated Hospital of Central South University Xiangya School of Medical, Haikou, Hainan, 570208, P. R. China.

Scientific reports

|July 2, 2025

概括

脂肪基衍生干细胞 (ADSCs) 增强了膀癌的进展. 与ADSCs长时间的共同培养可以提高BLCA细胞中的癌症干细胞,耐药性和免疫逃避,这表明在瘤微环境中具有前性作用.

科学领域:

在瘤学瘤学.
干细胞生物学干细胞生物学
癌症微环境癌症微环境

背景情况:

脂肪衍生干细胞 (ADSC) 在膀癌 (BLCA) 进展中的作用尚不清楚.
研究ADSCs对BLCA细胞入侵,干细胞,上皮-介质细胞转换 (EMT) 和耐药性的影响至关重要.

研究的目的:

为了确定与ADSCs长期共同培养对BLCA细胞特征的影响.
阐明ADSCs影响BLCA进展的机制,包括干性,耐药性和免疫逃逸.

主要方法:

在不同比例 (10:1, 3:1, 1:1) 的ADSCs与BLCA (T24) 细胞共培养14天.
使用CCK-8,Transwell,伤口愈合和流式细胞计量试验评估细胞增殖,入侵,迁移和细胞亡.
分析基因和蛋白质表达通过RNA测序,qRT-PCR和西部血栓;使用特定抑制剂评估的耐药性和干性 (Atezo, CP-31,398).

主要成果:

与ADSCs长期共同培养显著增强BLCA细胞的干细胞和耐药性.
同培养的T24细胞表现出增加的增殖,入侵,EMT,干性,耐药性和免疫逃逸,由上调的MDM2,mt-P53和PD-L1.1调节.
针对mt-P53和PD-L1的抑制剂逆转了在T24细胞中观察到的耐药性和干细胞.

结论:

在膀癌瘤微环境中长期存在的ADSCs起着前致癌作用.
ADSCs通过增强癌细胞干细胞,耐药性和免疫逃避来促进BLCA的进展.
针对ADSC介导的途径可能为膀癌提供新的治疗策略.

关键词:

脂肪酸衍生型的 stromal 干细胞膀癌是一种癌症.药物耐药性药物耐药性药物耐药性表皮介质介质过渡的表皮介质.这是入侵的入侵.一个的茎.

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