相关实验视频

Updated: Jul 24, 2026

Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System

Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System

Published on: January 31, 2018

在Bacillus subtilis上对仿真免疫原体的排序介导表面显示:一个死肠炎候选疫苗候选人

Bassima Al-Aneed^1,2, Hoang Duc Nguyen³, Ali Akbar Masoudi⁴

¹Department of Animal Science, Faculty of Agriculture, Tarbiat Modares University, P.O. Box 14115-336, Pajoohesh Blvd, Tehran, 149771311, Iran.

Microbial cell factories

|July 2, 2025

概括

在Bacillus subtilis上显示的一种新型重组化学蛋白 (R-anz) 显示出作为对的死肠炎疫苗的承诺. 口服免疫提高了抗体和细胞因子水平,提供了对Clostridium perfringens的部分保护.

科学领域:

蛋白质工程是一种蛋白质工程.
疫苗的研发工作正在进行中.
禽类免疫学禽类免疫学

背景情况:

死亡性肠炎 (NE) 对家禽健康和家禽产业构成重大威胁.
开发针对Clostridium perfringens的有效疫苗对于可持续的家禽养殖至关重要.

研究的目的:

设计和评估一种新型重组化学蛋白 (R-anz) 以作为一种针对NE的活细菌疫苗的潜在用途.
评估R-anz疫苗在中的免疫性和保护性有效性.

主要方法:

用排序酶介导的表面显示技术来表达Bacillus subtilis上的R-anz.
进行了生物信息学对接,以预测与的Toll-like受体21 (TLR21) 的相互作用.
蛋白质表达通过SDS-PAGE和Westernblotting得到证实;免疫性通过ELISA和细胞因子分析进行评估;有效性通过挑战研究进行评估.

主要成果:

在B. subtilis.上成功表达和显示R-anz化学蛋白.
口服免疫诱导了显著的IgY抗体产生和调高了关键细胞因子 (IFN-γ,IL-4,IL-17,IL-22).
接种疫苗的在挑战后显示肠道病变得分有所降低,这表明对C. perfringens的部分保护.

结论:

R-anz 嵌合蛋白疫苗可以激活的幽默性免疫和细胞免疫.
这种新型疫苗策略显示了减轻死肠炎和改善家禽健康的潜力.
需要进一步的研究来优化有效性,并探索可持续家禽管理的商业可行性.

关键词:

这种细菌是 Bacillus subtilis.奇美蛋白质疫苗的疫苗克洛斯特里 (Clostridium perfringens) 破坏了毒素和毒素.蛋白质工程是一种蛋白质工程.通过排序介导的表面显示器显示.疫苗的研发工作正在进行中.

更多相关视频

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes

Published on: December 19, 2020

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

相关概念视频

Antibiotic Selection

Antibiotic Selection

Bacterial Gastroenteritis

Bacterial Gastroenteritis

Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid receptor...