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通过纳米孔的基因组学:一种低成本的基因组学方法

Christina Meiring^1,2, Monique Eygelaar^1,2, Josephus Fourie³

¹Clinglobal, B03/04, The Tamarin Commercial Hub, Tamarin, 90903, Mauritius.

|July 2, 2025

概括

这项研究展示了一种可负担的方法,使用牛津纳米孔测序生成牛基因组. 这种方法使低资源的实验室能够进行基因组学,产生高质量的Rhipicephalus microplus和Rhipicephalus appendiculatus基因组.

科学领域:

基因组学就是基因组学.
生物信息学是一种生物信息学.
寄生虫学的寄生虫学

背景情况:

基因组组装通常很昂贵,需要多种测序技术和高性能计算.
这可能会导致对外部服务提供商的依赖,限制某些机构的可访问性.

研究的目的:

为了独立生成牛Rhipicephalus microplus和Rhipicephalus appendiculatus的基因组草案.
用牛津纳米孔测序技术展示一种可访问和具有成本效益的方法.

主要方法:

使用牛津纳米孔测序专门用于数据生成.
使用Shasta组装和抛光基因组在亚马逊网络服务云平台上,有折扣的Spot实例.
使用纳米孔Q20+库套件和流细胞对富于AT的线粒体DNA进行了测序.

主要成果:

生产的R. microplus和R. appendiculatus基因组与使用更昂贵的方法生产的基因组相当.
预测了R. appendiculatus的52,412个基因 (31,747个注释) 和R. microplus的60,935个基因 (32,263个注释).
组装和注释了独特的Coxiella类内共生生物基因组,并注意到两种物种的基因组缩小.

结论:

对于预算有限的实验室来说,它是一个可行的替代方案,可以参与基因组学.
在没有大量资本投资的情况下,使基因组学能力建设成为可能.
促进参与基因组研究,通常需要大量资金或合作.

关键词:

云计算,的基因组在新的一次.基因组组装组的基因组组装组长时间阅读阅读一个纳米孔.

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