从传统的意大利奶酪制造中分离的乳球菌 I7:一个生物技术综合平台
Alberto Alfano1, Darshankumar Parecha2, Alessandra Fusco2
1Department of Experimental Medicine, University of Campania Luigi Vanvitelli, Napoli, Italy. alberto.alfano@unicampania.it.
BMC biotechnology
|July 2, 2025
概括
研究人员优化了适用于乳球菌的素食媒介,显著提高了生物质和乳酸生产. 由此产生的抗微生物化合物有效地降低了病原体的生存能力,显示了食品安全应用的潜力.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
背景情况:
- 从传统来源对乳酸细菌越来越感兴趣.
- 从手工奶酪乳酪培养物中分离的乳酪菌.
- 传统发酵乳制品对健康有好处.
研究的目的:
- 优化素食媒介用于乳球菌乳酸菌的培养.
- 评估生长能力和代谢指纹.
- 提高生物质和生物活性分子的生产.
主要方法:
- 在优化素食媒介中进行料批发发酵.
- 设计实验方法用于媒体优化.
- 基于膜的下游加工 (微过/超过).
主要成果:
- 达到了14 g/L干细胞重量和1.9x10^10 CFU/mL.
- 乳酸最大产量为78g/L (增加五倍).
- 针对Enterococcus faecalis,沙门氏菌Typhimurium和大肠杆菌的抗菌活性 (6-9日志减少).
结论:
- 料批量和微过工艺增强了生物质和生物活性分子的生产.
- 优化的素食媒体支持强大的乳球菌乳腺菌生长.
- 获得的生物活性分子对病原体具有显著的抗微生物特性.
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