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构建和评估复合的植物乳杆菌表达Micropterus salmoides hepcidin的植物乳杆菌

Cheng-Long Zhang^1,2, Guangtao Chen^1,2, Xiang Shan Ji^1,2

¹Key Laboratory of Efficient Utilization of Non-grain Feed Resources (Co-construction by Ministry and Province) of Ministry of Agriculture and Rural Affairs, Shandong Agricultural University, Taian, Shandong, China.

Microbial cell factories

|July 2, 2025

概括

这项研究设计了Lactobacillus plantarum (LP37) 来表达Micropterus salmoides hepcidin (MsHep),增强鱼类的抗病能力和生长. 重组LP37-MsHep显示出作为绿色水产养殖料添加剂的前景.

科学领域:

水产养殖是水产养殖的一种方式.
微生物学微生物学
免疫学免疫学免疫学

背景情况:

肝素是先天免疫的关键抗微生物,对养殖动物的抗病能力至关重要.
乳酸菌植物 (LP37) 是一种有益的益生菌,以肠道殖民和高效的外来蛋白质表达而闻名.
之前的研究使用LP37促进鱼类的生长,如Micropterus salmoides.

研究的目的:

为了构建一个复合的植物乳杆菌 (LP37) 菌株表达Micropterus salmoides hepcidin (MsHep).
评估复合LP37-MsHep在增强抗菌能力和促进M. salmoides.生长中的有效性.

主要方法:

利用pSIP403表达向量与TUFA促进剂以及用于细胞外MsHep表达的分泌信号.
通过转化构建了重组菌株LP37-MsHep.
评估了对Aeromonas hydrophila和其他微生物的抗菌活性.
补充了M. salmoides饮食的LP37-MsHep60天,并评估了生存率,细菌负载,免疫基因表达和生长参数.

主要成果:

重组LP37-MsHep对Aeromonas hydrophila表现出显著的抗菌活性.
食补充剂改善了M. salmoides的存活率,并减少了感染后的细菌负载.
与铁代谢和免疫相关的基因表达 (FPN1,IL-10,TNF-α,MHC-II) 被调节.
增长表现得到改善,最终体重增加,体重增加比率,特定增长率和料转换率降低证明了这一点.

结论:

重组LP37-MsHep有效地改善了M. salmoides的生长性能和抗病能力.
这种工程益生菌是一种有希望的,环保的水产养殖料添加剂,具有广泛的应用潜力.

关键词:

乳酸菌植物植物 (Lactobacillus plantarum) 是一种植物菌.麦克罗普特鲁斯·萨尔莫伊德斯 (Micropterus salmoides) 是一个很小的动物.抗微生物是一种抗菌.肝素是一种肝素.再组合菌株是一种重组菌株.

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