使用猪的基因组和表观基因组特征对猪变异的多维注释
Hongwei Yin1, Liu Yang1, Qianyi Zhao1
1Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi-Omics of MARA, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, CAAS, Building A, Room 311, Buxin Road, Dapeng, Shenzhen, 518124, China.
BMC biology
|July 2, 2025
概括
这项研究对猪基因组变异进行了分类,揭示了功能丧失和非编码变异对基因表达,拼接以及背部脂肪厚度等复杂特征的影响.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 动物科学动物科学
背景情况:
- 了解基因组变异的影响对于复杂的特征机制至关重要.
- 收集了来自不同品种猪的1817个全基因组序列的数据集.
研究的目的:
- 为了对猪的基因组变异进行分类.
- 阐明变异对表观基因组,转录基因组和复杂特征的功能后果.
主要方法:
- 在1817头猪的全基因组测序.
- 功能丧失和非编码变体的识别和注释.
- 深度学习 (Basenji) 和ATAC-seq用于预测变异对染色质可访问性的影响.
主要成果:
- 确定了27,167种影响基因表达和拼接的功能丧失变异.
- 在5'UTR中非编码变异影响了基因表达,拼接和复杂的特征.
- 预测的变异对染色质可访问性的影响与对基因表达和特征的影响相关,特别是背部脂肪厚度.
结论:
- 创建了一个关于猪基因组变异 (编码和非编码) 的全面目录.
- 这些变异对表观基因组,转录基因组和复杂特征的功能后果得到了阐明.
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