多组学和机器学习识别免疫代谢生物标志物,用于对非结核菌和潜伏结核感染的活跃结核病诊断
Nguyen Tran Nam Tien1, Nguyen Thi Hai Yen1, Nguyen Ky Phat1
1Department of Pharmacology and PharmacoGenomics Research Center, Inje University College of Medicine, Busan 47392, Republic of Korea.
这项研究使用机器学习识别了新的脂质生物标志物,以区分活跃结核病 (TB) 与非结核菌 (NTM),潜伏结核感染 (LTBI) 和其他肺部疾病 (ODx). 脂质PC{14:0_22:6) 显示出作为结核病的诊断生物标志物的巨大潜力.
科学领域:
- 免疫代谢过程中的免疫代谢.
- 机器学习 机器学习
- 生物标志物发现发现
背景情况:
- 结核病 (TB) 与非结核菌 (NTM),潜在结核感染 (LTBI) 和其他肺部疾病 (ODx) 的准确区分在临床上具有挑战性.
- 循环生物标志物为疾病诊断和分类提供了一种非侵入性的方法.
研究的目的:
- 通过使用多组学和机器学习,识别和验证循环免疫代谢生物标志物,以区分活性结核病与NTM,LTBI和ODx.
- 优先考虑结核病的潜在诊断生物标志物候选者.
主要方法:
- 从发现和验证队列中收集了多组数据 (包括脂组数据).
- 基于机器学习的预测建模用于生物标志物识别和验证.
- 外部数据集被用于进一步独立验证已识别的生物标志物.
主要成果:
- 三种血多组生物标记有效地区分了活性结核病与非结核病的情况,AUC范围为0.70-0.90.
- 脂质PC ((14:0_22:6) 被确定为关键预测因子,在活跃的结核病患者中始终较低.
- 外部验证证实高疗效 (AUC 0.77-1.00) 在区分活跃结核病.
结论:
- 脂质代表了对结核病,NTM,LTBI和ODx的分类有前途的生物标志物.
- PC(14:0_22:6) 是活跃结核病差异诊断生物标志物的强有力的候选者.
- 这项研究证明了多组学和机器学习在识别复杂呼吸道感染的新型诊断标志物的力量.
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