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Updated: Apr 28, 2026

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

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通过单分子光解试验与目标循环CRISPR/Cas12a放大系统进行miRNA的快速和灵敏检测

Tengfang Zhang1, Leyan Cai1, Zibin Chu1

  • 1State Key Laboratory of Organic-Inorganic Composites, Beijing Key Laboratory of Bioprocess, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.

Small (Weinheim an der Bergstrasse, Germany)
|July 2, 2025
PubMed
概括

一种新的测定方法可以在几分钟内检测出具有高灵敏度的癌症微RNA (miRNA). 这种快速的方法显示了早期癌症诊断和个性化医学的前景.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?DNA纳米技术 DNA纳米技术消沉的消沉方式检测miRNA检测的方法单个分子光的光.

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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