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附加的III型分泌系统效应器集体塑造肠道炎症感染的结果
Priyanka Biswas1, Julia Sanchez-Garrido1, Zuzanna Kozik2
1Department of Life Sciences, Imperial College London, London, UK.
在Citrobacter rodentium的研究中,
科学领域:
- 微生物学 微生物学
- 病变的发生和发病.
- 细菌遗传学 细菌遗传学
背景情况:
- 第三类分泌系统 (T3SS) 对格拉姆阴性细菌感染至关重要.
- T3SS效应器,特别是辅助效应器的组织和作用尚未完全理解.
- 虫 (Citrobacter rodentium,简称CR) 是一种肠道病原体,用于研究T3SS效应器网络.
研究的目的:
- 在Citrobacter rodentium中剖析T3SS效应器网络.
- 区分核心和辅助效应因子以及它们在感染中的作用.
- 了解辅助效应器如何调节宿主反应和感染结果.
主要方法:
- 对CR T3SS效应器网络进行系统解剖.
- 产生和分析一个缺乏12个辅助因子 (CR_M12) 的CR菌株.
- 在感染期间对结肠上皮细胞进行深度蛋白质组分析.
- 在敏感和耐药小鼠模型中进行感染研究,包括Il22-/-小鼠.
主要成果:
- 确定了12个辅助的T3SS效应器,这些效应器对于殖民是不可或缺的,但改变了感染结果.
- 在耐药小鼠中,CR_M12导致表皮损伤,炎症和免疫细胞透减少.
- 蛋白质组分析显示,CR_M12感染改变了全球蛋白质表达和关键屏障完整性调节器,与野生型CR不同.
- 附加效应因素在没有显著影响毒性的情况下塑造感染结果,特别是在障碍修复受损的宿主中.
结论:
- 完善了对核心与辅助T3SS效应器的理解.
- 附加效应因子在调节宿主反应和感染严重程度方面发挥着重要作用.
- 提供了未来研究效应驱动宿主适应和毒性调节的基础.
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