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在老年小鼠中,miR-10a-5p和miR-10b-5p恢复了结肠运动性

Gain Baek¹, Rajan Singh¹, Se Eun Ha¹

¹Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, United States.

World journal of gastroenterology

|July 2, 2025

概括

老化肠道中的miR-10a-5p和miR-10b-5p水平下降会损害Cajal (ICC) 的间歇细胞功能,导致便秘. 在老年小鼠中恢复这些microRNA改善了胃肠道的运动性,这表明了潜在的治疗方法.

科学领域:

胃肠病学胃肠病学
分子生物学分子生物学
衰老研究研究衰老研究

背景情况:

微RNA-10b-5p (miR-10b-5p) 通过调节Cajal (ICC) 的间歇细胞,对胃肠 (GI) 运动至关重要.
与年龄相关的miR-10b-5p的下降有助于便秘.
在ICC中,miR-10a-5p与miR-10b-5p共同表达.

研究的目的:

研究miR-10a-5p和miR-10b-5p在与年龄相关的肠动性中的作用.
评估恢复miR-10a-5p和miR-10b-5p表达的治疗潜力.

主要方法:

使用了老年小鼠,miR-10a和miR-10b淘汰模型以及人类血/结肠样本.
评估的GI和结肠过境,ICC网络完整性,以及miR-10a/b-5p表达.
经过测试的microRNA模仿治疗,以恢复老年小鼠的胃肠道运动.

主要成果:

老年小鼠显示过渡延迟,便输出减少和miR-10a/b-5p表达减少,与ICC网络恶化相关.
淘汰赛模型显示运动能力受损和ICC损失;miR-10a KO小鼠具有更严重的表型.
在老年小鼠中,miR-10a-5p和miR-10b-5p模仿治疗显著改善了肠道运动性,并确认了肠道输送.

结论:

miR-10a-5p和miR-10b-5p对于ICC维护和结肠机动性至关重要.
这些microRNAs与年龄相关的下降有助于小鼠和人类的胃肠道不运动.
恢复miR-10a-5p和miR-10b-5p水平是对运动障碍的有希望的治疗策略.

关键词:

年龄大的小鼠.便秘便秘便秘便秘胃肠道运动不良.卡哈尔的间歇细胞.微RNAs是一种微RNA.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...