奥林克蛋白质组学揭示了TNFRSF9作为腹腔大动脉动脉瘤的生物标志物
Runze Chang1, Heng Wang1,2, Chuanlong Lu1
1The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.
iScience
|July 2, 2025
概括
腹腔大动脉动脉瘤 (AAA) 涉及炎症. 这项研究确定了中白素-6 (IL-6) 和瘤亡因子受体超级家族9 (TNFRSF9) 成员作为关键的炎症标志物,TNFRSF9显示为AAA的诊断生物标志物的潜力.
科学领域:
- 心血管研究研究心血管研究
- 蛋白质组学是指蛋白质组学.
- 炎症生物学 炎症生物学
背景情况:
- 腹腔大动脉动脉瘤 (AAA) 是一种严重的心血管疾病,其特征是慢性炎症.
- 识别可靠的炎症标志物对于了解AAA病原和诊断至关重要.
研究的目的:
- 利用奥林克蛋白质组学来发现与AAA相关的血清炎症标志物.
- 在AAA患者中调查已识别的炎症标志物的诊断潜力.
主要方法:
- 采用奥林克近距离延伸试验 (PEA) 来分析AAA患者和健康对照中的92种与炎症相关的蛋白质.
- 集成的奥林克蛋白质组学数据与基因表达数据 (GSE183464) 以及使用ELISA验证的结果.
- 使用接收器运行特征 (ROC) 曲线分析来评估诊断价值.
主要成果:
- 奥林克蛋白质组学发现了38种不同表达的蛋白质.
- 干白素-6 (IL-6) 和瘤亡因子受体超级家族成员9 (TNFRSF9) 在AAA中在基因和蛋白质水平上升调节.
- TNFRSF9和IL-6显示出高的诊断价值 (ELISA AUC=0.950). 这两种类型的药物具有较高的诊断价值.
- 在AAA的动物模型中证实了TNFRSF9表达的升高.
结论:
- 在AAA中,TNFRSF9和IL-6被显著上调,具有诊断潜力.
- TNFRSF9可以作为一个有前途的生物标志物来诊断腹腔大动脉瘤.
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