来自牛,土壤和肥土壤样本的111个元基因组组合基因组的数据集
Eduardo Pérez-Valera1,2, Dana Elhottová2
1INRAE, University Bourgogne, Institut Agro Dijon, Department of Agroecology, 17 rue de Sully, Dijon, 21000, France.
Data in brief
|July 2, 2025
概括
这项研究报告了来自肥料和土壤的111种高质量的非发酵格拉姆阴性细菌 (NFGNB) 的元基因组组装基因组 (MAG). 这些MAG提供了对NFGNB从抗生素处理的肥到农业土壤的分散的基因组洞察.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 环境科学 环境科学
背景情况:
- 用抗生素处理的动物便可以在农业土壤中引入非发酵格拉姆阴性细菌 (NFGNB).
- 了解NFGNB的基因组特征对于评估它们的环境分散和生态影响至关重要.
研究的目的:
- 从肥料和土壤微观宇宙中重建和描述NFGNB的元基因组组装基因组 (MAGs).
- 调查NFGNB从经过抗生素处理的奶牛便到牧场土壤的潜在传播.
主要方法:
- 使用CHROMagar Acinetobacter介质从肥料和土壤样本中丰富NFGNB.
- 使用Illumina NovaSeq 6000进行DNA测序,并使用metaSPAdes进行读取组装.
- 使用MetaBAT,MaxBin,SemiBin2,COMEbin和AVAMB进行MAG重建,然后使用dRep.de复制.
主要成果:
- 获得了111个中高质量的MAG,主要分配给Pseudomonadota (99个MAG),包括Pseudomonas, Stenotrophomonas和Acinetobacter.
- 主要来源于肥 (44),其次是肥土壤 (38) 和土壤 (29).
- 高质量的MAG在便样本中更频繁地被检测出来.
结论:
- 产生的MAG为研究NFGNB在土壤环境中的生存,分散和生态作用提供了有价值的基因组资源.
- 这一数据集对于了解经过抗生素处理的动物便对土壤微生物群落的影响尤为重要.
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