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评估和优化循环介导异热放大 (LAMP) 技术用于卡普里波克病毒诊断,并与PCR方法进行比较

M Edrisi1, H R Varshovi2, S Safi1

  • 1Department of Pathobiology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Poonak, Hesarak, Tehran, Iran.

Archives of Razi Institute
|July 2, 2025
PubMed
概括

一种新的循环介导同热放大 (LAMP) 测试提供了快速,经济高效的检测,影响羊,山羊和牛的病毒 (CaPVs). 这种高度敏感的方法与PCR精度相匹配,非常适合资源有限的环境.

关键词:
卡普里波克病毒 (Capripoxvirus) 是一种病毒.一个灯的灯光.在LSDVV中使用.这是一个PCRPCR.

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...