相关实验视频

Updated: Sep 17, 2025

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

结肠直肠癌中失调的长非编码RNA:使用RNA-seq和实时逆转录聚合酶链反应识别和验证

Reyhaneh Dehghanzad¹, Roghayeh Rahbar Parvaneh², Mohammad Keramatipour³

¹Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

|July 2, 2025

概括

这项研究确定了四种长非编码RNA (lncRNAs),它们在结直肠癌 (CRC) 组织中显著上调. 这些lncRNAs显示出作为CRC诊断和治疗的生物标志物的潜力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

结肠直肠癌 (CRC) 是一个主要的全球健康问题,在发病率上排名第三,在与癌症有关的死亡率上排名第二.
长非编码RNAs (lncRNAs) 越来越多地被认为在各种癌症的发展和进展中发挥着关键作用,包括CRC.
像RNA测序 (RNA-seq) 等高通量技术的进步为分析癌症奥米克数据和发现新型分子机制提供了强大的工具.

研究的目的:

识别和验证涉及结直肠癌 (CRC) 病变发生的新型长非编码RNA (lncRNAs).
探索已识别的lncRNAs作为CRC的诊断生物标志物或治疗点的潜力.

主要方法:

在癌症基因组图谱 (TCGA) 数据上进行了RNA测序 (RNA-seq),分析了481个CRC样本和41个健康对照.
博鲁塔特征选择算法确定了统计学上显著的差异表达的lncRNAs.
四个候选 lncRNAs (LINC01730,LINC02487,LINC01836和LINC01594) 被选择用于实时逆转录聚合酶链反应 (RT-PCR) 在10个CRC和9个正常组织样本上进行验证.
进行了生物信息分析,以调查与CRC中确定的lncRNA相关的分子途径.

主要成果:

RNA-seq分析揭示了388个在CRC和健康组织之间差异表达的lncRNA.
实时RT-PCR验证了四个lncRNAs的显著上调:LINC01730,LINC02487,LINC01836和LINC01594在CRC组织中.
生物信息分析表明,这些上调的lncRNAs可能参与调节瘤生长和转移的关键分子途径.

结论:

在结直肠癌 (CRC) 组织中鉴定和验证了四种明显上调的lncRNAs (LINC01730,LINC02487,LINC01836和LINC01594).
这些lncRNAs显示出潜力,作为开发新生物标志物和治疗CRC的治疗策略的有前途的候选人.
对这些lncRNAs的功能作用的进一步研究可以促进我们对结直肠癌的理解和治疗.

关键词:

结肠直肠癌是什么意思长非编码RNAs 长非编码RNA RNA测序分析的分析实时逆转录聚合酶连锁反应

更多相关视频

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Published on: July 22, 2020

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...