肠道病毒体中的偏差和互补性,通过散装和病毒样颗粒丰富的元基因组测序获得
Yun Li1, Chuqing Sun2, Jiaying Zhu1
1Key Laboratory of Molecular Biophysics of the Ministry of Education, Hubei Key Laboratory of Bioinformatics and Molecular Imaging, Center for Artificial Intelligence Biology, Department of Bioinformatics and Systems Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Microbiology spectrum
|July 2, 2025
概括
对肠道病毒组分析的批量和病毒样粒子 (VLP) 测序进行比较,发现VLP捕获了更完整的病毒基因组. 然而,这两种方法都是互补的,为肠道微生物群提供了独特的见解.
科学领域:
- 微生物学 微生物学
- 病毒学 病毒学
- 生物信息学是一种生物信息学.
背景情况:
- 肠道病毒组研究由于不同的测序策略而面临变化.
- 大量和病毒样粒子 (VLP) 丰富的元基因组测序具有明显的优势和局限性.
- 需要进行全面的比较,以了解肠道病毒组合和偏差.
研究的目的:
- 从配对的批量和VLP测序数据中获得的肠道病毒组进行全面比较.
- 确定批量和VLP测序策略之间的偏差和互补性.
- 为最佳肠道病毒组表征提供建议.
主要方法:
- 151个成年人和141个婴儿便样本的配对散装和VLP元基因组测序.
- 病毒基因组恢复,长度,完整性和分类注释的分析.
- 评估病毒社区结构,并确定每个方法中的偏差.
主要成果:
- VLP测序产生了更多,更长,更完整的病毒基因组,对低丰度病毒的敏感性更高.
- 在批量和VLP方法之间没有观察到病毒社区结构 (香农多样性) 的相关性,这表明了VLP丰富偏差.
- 病毒患病率低,丰度低或具有格拉姆阳性细菌宿主的病毒在VLP数据中得到了丰富.
- 发现了显著的互补性,两种方法之间的病毒基因组只有~27-29%的重叠.
结论:
- 在病毒基因组发现方面,VLP测序卓越,但引入了偏见,可能会改变社区结构.
- 大量测序提供了更稳定的社区形象,但可能会产生碎片化的基因组.
- 建议使用批量和VLP策略,以全面了解人类肠道病毒组.
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