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冷保存的人类精子的形态和功能分析:不同冷方案的比较

C Omes¹, M Savio², G Mazzini³

¹Center for Reproductive Medicine, Obstetrics and Gynecology Unit 2, Woman and Child Health Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. c.omes@smatteo.pv.it.

|July 2, 2025

概括

优化精子冷保存是保持生育能力的关键. 使用TEST-Yolk Buffer (TYB) 在+4°C的预冷步骤显著改善了解后的精液质量.

科学领域:

生殖医学生殖医学
精子生物学精子生物学
低温生物学低温生物学

背景情况:

精子冷保存对于在接受可能危及生育能力的治疗或患有阿佐精子症的患者中保持生育能力至关重要.
目前的冷保存方法缺乏标准化,影响了冷存活率.

研究的目的:

在五种经过测试的方法中确定最佳的精子冷协议.
评估基于常规分析和先进的测试,如彗星测试和电子显微镜.

主要方法:

分析了26个新鲜精液样本,使用了五种不同的冷方案.
使用的冷保护剂:TEST-Yolk缓冲器 (TYB).
评估参数:精子运动性,活力,膜完整性,DNA碎片化和超结构.

主要成果:

方法1和方法3采用了三相冷却过程,包括+4°C阶段和先前浸入液气中的方法,表现出优异的结果.
这些协议最大限度地减少了融化后的精液质量恶化.

结论:

在使用TYB进行精液冷保存时,在+4°C的冷前阶段至关重要.
使用多种测定 (运动性,活力,DNA完整性,超结构) 的全面评估对于评估冷保存的有效性至关重要.

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