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Updated: Sep 17, 2025

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在病原性感染期间,对安瑟雷亚亚萨曼西斯 (Lepidoptera: Saturniidae) 的RT-qPCR分析稳定基因的评估

Indumathi Kamatchi Balakrishnan1,2, Himanshu Dubey3, Pawan Shukla1

  • 1Seribiotech Research Laboratory, Central Silk Board, Kodathi, Bangalore, India.

Molecular biology reports
|July 2, 2025
PubMed
概括

这项研究确定了最稳定的基因参考基因,用于在致病性压力下对Muga丝虫 (Antheraea assamensis) 进行准确的基因表达分析. 推RPS3A作为反转录定量PCR正常化最可靠的基因.

关键词:
这种植物是Antheraea assamensis.基因表达 基因表达 基因表达家庭管理基因免疫反应的免疫反应.

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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