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相关概念视频

Spermatogenesis01:41

Spermatogenesis

103.5K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
103.5K

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相关实验视频

Updated: Sep 17, 2025

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye
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Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

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关于精子发生的空间转录分析.

Ndifereke Uboh1, Sean Vargas2, Victoria D Diaz1

  • 1Department of Neuroscience, Developmental and Regenerative Biology, The University of Texas at San Antonio, San Antonio, TX, USA.

Methods in molecular biology (Clifton, N.J.)
|July 2, 2025
PubMed
概括
此摘要是机器生成的。

空间转录学揭示了丸中的细胞变异,克服了传统方法的局限性. 这项研究详细介绍了 Seeker (SlideSeq V2) 的应用,用于在小鼠丸中进行空间基因表达分析.

关键词:
生物信息学是一种生物信息学.在RNA-seqqq.塞拉特·塞拉特是什么意思空间转录组学 空间转录组学组织截面 组织截面

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Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis
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Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis

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A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
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A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

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相关实验视频

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A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 发展生物学 发展生物学

背景情况:

  • 传统的转录学具有破坏性,缺乏空间上下文.
  • 空间转录学保存组织架构用于细胞异质性分析.
  • 精子生成表现出空间变异,这对于解释基因表达数据至关重要.

研究的目的:

  • 提供空间转录组技术的概述.
  • 为在丸中应用Seeker (SlideSeq V2) 空间转录组学提供指导方针.
  • 在小鼠丸中演示空间转录基因数据的生物信息分析.

主要方法:

  • 空间转录组技术的概述.
  • 在丸组织上应用Seeker (SlideSeq V2) 的详细协议.
  • 生物信息分析管道用于空间基因表达数据.

主要成果:

  • 在小鼠丸中展示空间基因表达分析.
  • 识别与精子生成阶段相关的空间模式.
  • 使用空间转录组学对圆形精子体的示例分析.

结论:

  • 空间转录学对于理解诸如精子生成之类的空间组织生物过程至关重要.
  • 搜索器 (SlideSeq V2) 提供了一个强大的工具,用于高分辨率的空间基因表达分析在丸.
  • 整合空间上下文对于解释复杂的转录数据集至关重要.