产生和使用人类iPSC衍生的含有微质的空气-液体界面皮质有机体培养的协议
Marta Cañizares Luna1, Mayte Mars1, Channa E Jakobs1
1Department of Translational Neuroscience, UMC Utrecht Brain Center, University Medical Center Utrecht, Utrecht University, Utrecht 3584 CG, the Netherlands.
STAR protocols
|July 2, 2025
概括
我们开发了一种长期含有微质的有机体培养的协议,可以减少细胞死亡. 这种新方法支持微质的存活和恒温,用于在3D大脑模型中研究神经免疫相互作用.
科学领域:
- 神经科学是一个神经科学.
- 免疫学 免疫学 免疫学
- 干细胞生物学 干细胞生物学
背景情况:
- 扩展的有机体培养物经常患有死核形成,这限制了它们对研究长期细胞过程的有用性.
- 微质细胞,大脑的常驻免疫细胞,对于神经发育和疾病至关重要,但它们在传统的有机体模型中生存是具有挑战性的.
研究的目的:
- 提出一种用于产生含有微质的空气-液体界面皮质器官 (MG-ALI-COs) 的新方案,能够进行长期培养.
- 在3D大脑模型中克服死核形成的局限性,增强微质存活率和恒常性.
- 为研究人类神经免疫相互作用提供一个生理相关的平台.
主要方法:
- 产生空气-液体界面皮质器官 (ALI-COs).
- 将巨细胞前体集成到ALI-COs中以产生MG-ALI-COs.
- 维护和长期培养MG-ALI-COs.
- 实验分析包括免疫染色,成像和补丁电生理学.
主要成果:
- 该协议成功地产生了长期的MG-ALI-CO培养.
- 尽量减少死核的形成,促进微质细胞的存活和平衡.
- 证明了MG-ALI-COs在各种实验分析中的实用性.
结论:
- 开发的MG-ALI-CO模型提供了一个强大的系统,用于在3D,类似大脑的环境中研究小质细胞.
- 这种模型有助于长期研究人类神经免疫相互作用.
- 该协议解决了神经免疫学研究当前有机体技术的关键局限性.
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