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光学笔切割显微镜用于3D成像和悬浮细胞操纵

Xing Li^1,2, Dan Dan¹, Siarhei Zavatski³

¹State Key Laboratory of Ultrafast Optical Science and Technology, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.

Science advances

|July 2, 2025

概括

这项研究介绍了光学 tweeze-sectioning 显微镜,一种全光学方法用于悬浮细胞的3D成像. 它将结构化照明显微镜 (SIM) 与全息光学 tweezers (HOT) 结合起来,用于精确的操纵和成像.

科学领域:

生物光子学生物光子学
光学显微镜的使用方法
细胞成像细胞成像

背景情况:

光学切割 (OS) 对3D显微镜至关重要,但传统上需要非光学方法,如样品粘附或机械扫描.
这些局限性阻止了使用OS技术对不粘附的悬浮细胞进行成像.

研究的目的:

开发适用于悬浮电池的光学切割 (OS) 的全光学解决方案.
为了使非粘附生物颗粒的3D显微镜.

主要方法:

连接结构化照明显微镜 (SIM) 与全息光学子 (HOT).
在HOT中利用雕塑光来减少悬浮细胞的位置波动,以纳米精度.
通过细胞的光学输送实现样本扫描,消除了对机械翻译阶段的需求.

主要成果:

演示了光学子切割显微镜,SIM和HOT的集成.
通过使用光,成功地实现了对悬浮酵母细胞位置的纳米级控制.
通过全光学方法启用了悬浮细胞的光学切割和3D成像.

结论:

介绍了一种用于悬浮电池的光学切割 (OS) 的全光学新方法.
将OS显微镜的实用性扩展到不粘附的生物样本.
提供了一种独特的技术,通过SIM将3D成像与通过HOTS的光学操纵相结合.

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