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在中毒病例中的血样本中使用HybridSPE沉和LC-MS/MS快速确定酸

Juliana Blume de Oliveira¹, Bruno Pereira Dos Santos², Viviane Cristina Sebben³

¹Graduate Program in Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.

Legal medicine (Tokyo, Japan)

|July 2, 2025

概括

一种新的LC-MS/MS方法准确量化了血中的酸 (VPA),用于紧急毒理学. 这种快速分析有助于管理VPA过量服用病例,改善患者的治疗结果.

科学领域:

临床毒理学临床毒理学
分析化学分析化学
药理学药理学是指药理学的学科.

背景情况:

酸 (VPA) 是一种重要的抗药物,有可能导致毒性过量服用.
过量服用可导致严重的神经和器官功能障碍,需要快速识别.
精确量化血中的VPA对于及时的患者管理至关重要.

研究的目的:

在紧急毒理学中开发和验证用于VPA分析的快速LC-MS/MS方法.
在人体血样本中建立可靠的测定方法来量化VPA.
确保方法性能符合严格的临床应用验证指南.

主要方法:

液体染色学-并联质谱学 (LC-MS/MS) 用于VPA量化.
混合SPE-PPT技术用于用100μL血制备血样本.
方法验证遵循ANSI/ASB标准036指南的建议.

主要成果:

经过验证的方法实现了20μg/mL的下限量化值.
证明直线性高达500μg/mL (r2>0.999),具有高精度 (CV%<9%) 和准确性 (偏差在-10.4%内).
通过HybridSPE-PPT实现了快速的4分钟运行时间和>90%的恢复.

结论:

成功开发并验证了一种快速,精确和准确的LC-MS/MS方法,用于在血中量化VPA.
该方法适用于紧急毒理学场景,有助于管理VPA中毒.
这种测试为改善过量病例患者预后提供了关键的工具.

关键词:

这种混合SPE-PPTT.这是LC-MS/MS.这是中毒的毒害.瓦尔酸是一种酸.

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