一种基于双功能金纳米粒子的新型色度检测,与机器学习和深度学习模型相结合,用于识别食品中的微生物转质氨酶
Shihong Li1, Xia Liu1, Xu Geng1
1Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Sciences, Jilin University, 2699 Qianjin Street, Changchun, 130012, China.
Talanta
|July 2, 2025
概括
使用金纳米粒子和人工智能的新方法可以准确地检测食物中的微生物转质氨酶 (mTG). 这一进步有助于识别过度的mTG,防止与过度使用加工食品有关的潜在健康问题.
科学领域:
- 食品科学 食品科学 食品科学
- 分析化学 分析化学
- 生物技术是生物技术.
背景情况:
- 微生物转质氨酶 (mTG) 增强了食物的质感,但过度使用与健康问题有关.
- 目前在食品中的mTG检测方法有限.
研究的目的:
- 开发一种新的,敏感的方法来检测食品中的mTG活性.
- 使用金纳米粒子 (AuNPs) 和机器学习 (ML) 进行mTG量化.
主要方法:
- 使用双功能AuNP进行mTG活动的色度检测.
- 创建数据集,包含6种食物类型的648个mTG度-吸收点.
- 应用ML算法 (DT,RF,MLP) 进行预测.
主要成果:
- 在AuNP色度测量方法检测mTG从0.01U到1U高灵敏度.
- 多层感知器 (MLP) 模型实现了0.96的预测准确度.
- 盲目测试证实了在各种食品中mTG水平的准确预测.
结论:
- 本研究提出了一种有效的策略,用于识别和预测加工食品中的mTG活性.
- 开发的方法为食品安全和质量控制提供了敏感和准确的方法.
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