在整个COVID-19症状阶段解码血清N-Glycome签名
Mehmet Emrah Yaman1, Haci Mehmet Kayili2, İzzet Avci3
1Faculty of Pharmacy, Department of Analytical Chemistry, Ataturk University, Erzurum, Türkiye.
概括
研究人员在血液中确定了特定的N-甘氨酸结构,可以帮助诊断COVID-19并预测疾病的严重程度. 糖甘形状的变化为早期风险评估提供了潜在的生物标志物.
科学领域:
- 生物化学 生化学
- 免疫学 免疫学 免疫学
- 格莱科米克斯 (Glycomics) 是一个有趣的药物.
背景情况:
- 严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 感染具有不同的临床结果.
- 对于早期风险分层和COVID-19的严重程度预测,仍然需要可靠的生物标志物.
研究的目的:
- 鉴定COVID-19患者血清N-葡萄糖的特征,症状严重程度和健康对照的不同.
- 为了确定潜在的N-甘氨酸生物标志物用于COVID-19诊断和严重程度评估.
主要方法:
- 血清样本被酶去糖化.
- 使用水友互动液态染色学与光检测结合四极飞行时间质谱 (HILIC-FLD-QTOF-MS) 来分析N-糖.
- 进行了病例对照比较和接收器操作特征 (ROC) 分析.
主要成果:
- 与对照人群相比,COVID-19患者的寡甘和混合型甘含量下降,四天线和四银基结构增加.
- 四个N-glycan结构显示出诊断潜力,可以区分COVID-19和健康状态.
- 严重症状的COVID-19患者表现出天性基化N-甘氨酸 (SLex) 的增加.
- 一种Hex5HexNAc4Neu5Ac的异构体显示出严重的COVID-19的强烈诊断潜力 (AUC>0.8).
结论:
- 血清N-葡萄糖分析可以区分COVID-19患者和健康个体.
- 特定的甘氨酸变化,包括天线化和Hex5HexNAc4Neu5Ac异构体,与严重的COVID-19有关.
- 这些发现突出了COVID-19风险评估和严重程度预测的潜在新生物标志物.
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